Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.188609 |
Chromosome: | chromosome_16 |
Location: | 1354697 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g651850 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTCAGCGGCATCGGATGCTTCCCCCTTGCC |
Internal bar code: | CCGGAGTGCCGGATCTCAGATT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 819 |
LEAP-Seq percent confirming: | 99.7708 |
LEAP-Seq n confirming: | 1741 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGACGGCGACTTCTTCTTC |
Suggested primer 2: | GGCTCTTGGTTATCAGCTCG |