Insertion junction: LMJ.RY0402.188621_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.188621
Chromosome:chromosome 14
Location:3604968
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g631150 CEP14 (1 of 1) 2.4.2.26//2.7.11.1 - Protein xylosyltransferase / Uridine diphosphoxylose-protein xylosyltransferase // Non-specific serine/threonine protein kinase / Threonine-specific protein kinase; Cysteine endopeptidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAGCCGCATCAACATGAACGGTCGCTTTG
Internal bar code:GACTCGACCAAGTAGTGTGCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:394
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:558
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CATGACTGTTGATTGCCCAC
Suggested primer 2:ACGCGCATACTCACAGACAC