| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.188631 |
| Chromosome: | chromosome 12 |
| Location: | 4835759 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTACGGCGCCGCCAGCACATTGCCCGTC |
| Internal bar code: | GTGGCTGCAGGGATAGCCGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1024 |
| LEAP-Seq percent confirming: | 93.3184 |
| LEAP-Seq n confirming: | 4986 |
| LEAP-Seq n nonconfirming: | 357 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTTCACTCGTTTAGGCTC |
| Suggested primer 2: | CCGTGCTTAGACAGACACGA |