Insertion junction: LMJ.RY0402.188632_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.188632
Chromosome:chromosome 6
Location:3512690
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g278107 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACGACGCTGTGCGGTTGCGCTCTTGTTGC
Internal bar code:AAGCCCTGAGTAGGAGGGAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:109
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:643
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCCCATAACTAGCCCTAT
Suggested primer 2:AGGTGAGTCCATTTGTTGGC