Insertion junction: LMJ.RY0402.188658_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.188658
Chromosome:chromosome 13
Location:2790966
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre13.g582800 (1 of 1) 6.3.2.11 - Carnosine synthase / Carnosine synthetase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAAGAAGTCGGCTCTTAGTGCTGCTCTCG
Internal bar code:GAAGGTTCTAGGGTAAAGATAA

Confirmation - LEAP-Seq

LEAP-Seq distance:36
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:270
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TCATGCCATGGTAGCCATTA
Suggested primer 2:TGCACCAGCTTCATCTTGTC