Insertion junction: LMJ.RY0402.188711_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.188711
Chromosome:chromosome 10
Location:977644
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g424775 CTP2,PCH1,PAA1 Copper transporting ATPase and Cu chaperone for CTP1; (1 of 1) PTHR24093:SF124 - COPPER-TRANSPORTING ATPASE PAA1, CHLOROPLASTIC CDS|outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGCGCGCTCTCGTCCACACTGCTGCGGCC
Internal bar code:TTAGGTTCGATGAATGAGGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:520
LEAP-Seq percent confirming:99.1525
LEAP-Seq n confirming:117
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGGCTAGGAAGCTGAGAGGT
Suggested primer 2:AGACATGTGCGTGGTTGTGT