| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.188715 |
| Chromosome: | chromosome 7 |
| Location: | 2292883 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g327800 | (1 of 2) K05681 - ATP-binding cassette, subfamily G (WHITE), member 2 (ABCG2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCAGCACCTGCGATCATGTCAAACGCGC |
| Internal bar code: | GGGAGATTCGGGGGGCACCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 492 |
| LEAP-Seq percent confirming: | 99.9272 |
| LEAP-Seq n confirming: | 2745 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTACAGCATTCGTGCCTTG |
| Suggested primer 2: | GGTTGTAAAAACCAGCCCAA |