| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.188731 |
| Chromosome: | chromosome 6 |
| Location: | 5199058 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g281800 | (1 of 6) PF09353 - Domain of unknown function (DUF1995) (DUF1995) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGATGCGAGAGTGGCGCCAATCGGCTGG |
| Internal bar code: | CGAAGGAAAAGTTTGTGTTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 32 |
| LEAP-Seq percent confirming: | 98.9691 |
| LEAP-Seq n confirming: | 96 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTGCCAGTGTAGCGTTGG |
| Suggested primer 2: | TTTTGGCGGCTATCAATTTC |