| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.188742 |
| Chromosome: | chromosome 7 |
| Location: | 1939681 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325748 | (1 of 1) 3.5.2.9 - 5-oxoprolinase (ATP-hydrolyzing) / Pyroglutamase (ATP-hydrolyzing) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTAACCCCCCGCCTCGCCTCACTGTTCT |
| Internal bar code: | GGTGGTCCGGTGCTTAAGTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 398 |
| LEAP-Seq percent confirming: | 74.2474 |
| LEAP-Seq n confirming: | 13072 |
| LEAP-Seq n nonconfirming: | 4534 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCTTACCTATCCCGCCTG |
| Suggested primer 2: | CACTTGATGTGGGGTGTGAG |