| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.188743 |
| Chromosome: | chromosome_7 |
| Location: | 4754625 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g345400 | FAP70 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGACATGATTGCAATACATGGTGAAGACG |
| Internal bar code: | GTGAAGGCCATCGGGGATCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 839 |
| LEAP-Seq percent confirming: | 94.7984 |
| LEAP-Seq n confirming: | 3973 |
| LEAP-Seq n nonconfirming: | 218 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCAAGACGTCTGCCTACC |
| Suggested primer 2: | GCTACGAAGGAAAGCACAGG |