| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.188768 |
| Chromosome: | chromosome 16 |
| Location: | 5627506 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679650 | (1 of 1) PTHR19961:SF18 - FI19014P1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGACGGTAAGCACATTACGCCAACAAGAA |
| Internal bar code: | ACCCGGGAGTGGAGTAAGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 91 |
| LEAP-Seq percent confirming: | 80.2326 |
| LEAP-Seq n confirming: | 69 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGCATCCCCTAGTCTGCT |
| Suggested primer 2: | TGTCGGAGCATAACAAGCTG |