Insertion junction: LMJ.RY0402.188781_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.188781
Chromosome:chromosome 11
Location:1788891
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre11.g467450 SOM3 S-adenosyl-L-methionine-dependent O-methyltransferase; (1 of 4) PTHR13600//PTHR13600:SF12 - LEUCINE CARBOXYL METHYLTRANSFERASE // LEUCINE CARBOXYL METHYLTRANSFERASE CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCACCCCAAAACCAACTAGAACGAGCGG
Internal bar code:GTCTCGACTCGACTCCCGCTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:210
LEAP-Seq percent confirming:99.9129
LEAP-Seq n confirming:1147
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACTACAAGCCCACCAACCAG
Suggested primer 2:TACACACCACACACCACACG