| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.188790 |
| Chromosome: | chromosome 11 |
| Location: | 2077085 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g468900 | FAP404 | Flagellar Associated protein 404 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAACGCTTGCCGCGTACAAGGAAATCCC |
| Internal bar code: | CACCGATCGTTTCCATTAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 405 |
| LEAP-Seq percent confirming: | 99.8172 |
| LEAP-Seq n confirming: | 1638 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGCATGGTTGTTGAAGTG |
| Suggested primer 2: | TGGAGCACCCCTACCACTAC |