Insertion junction: LMJ.RY0402.188797_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.188797
Chromosome:chromosome 6
Location:5172387
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g281526 (1 of 1) IPR001660//IPR002048//IPR011011//IPR013761 - Sterile alpha motif domain // EF-hand domain // Zinc finger, FYVE/PHD-type // Sterile alpha motif/pointed domain 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTCGTGAAGTCTGTCCCAGCCTGCGCCA
Internal bar code:TTTAAGGCGCTTCATTGTTCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:570
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:347
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGTTGCTGATGTTGCTGT
Suggested primer 2:TCCTCTTCAACACAATCCCC