| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.188799 |
| Chromosome: | chromosome 16 |
| Location: | 631534 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692000 | (1 of 9) 3.1.3.2 - Acid phosphatase / Phosphomonoesterase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAATGCGGAATCGTGGGTAAAGAAGACTT |
| Internal bar code: | CGGCGCGGACCAACCTTGACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 993 |
| LEAP-Seq percent confirming: | 99.0645 |
| LEAP-Seq n confirming: | 5295 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCGTGTCATTAAGTCCGC |
| Suggested primer 2: | ATCTGGCAGAATAACCGTGG |