| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.188817 |
| Chromosome: | chromosome 16 |
| Location: | 1962527 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656400 | SQD1 | (1 of 1) 3.13.1.1 - UDP-sulfoquinovose synthase / Sulfite:UDP-glucose sulfotransferase; UDP-sulfoquinovose synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCGCACCGACACCCTGCCCTACCCCAAG |
| Internal bar code: | TTATCTTTTGAAAGTTGTTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 104 |
| LEAP-Seq percent confirming: | 79.2 |
| LEAP-Seq n confirming: | 99 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACTCATCCCAACAGACC |
| Suggested primer 2: | CATAGCACTATTCAGCGCCA |