Insertion junction: LMJ.RY0402.188834_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.188834
Chromosome:chromosome 1
Location:5310599
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g037150 CLV6 (1 of 1) PTHR11689//PTHR11689:SF14 - CHLORIDE CHANNEL // CHLORIDE CHANNEL PROTEIN 2; ion channel/ voltage-gated chloride channel intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGGGCAGTACGGCGACGACCACCGTCCC
Internal bar code:TAGGCCAGACGTGACTCGACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:360
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1020
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:ATTCACAGGTACTGTCGCCC
Suggested primer 2:ATCAGCGTACATACTCCGCC