Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.188840 |
Chromosome: | chromosome_1 |
Location: | 3651386 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g023550 | FAP234 | Flagellar Associated Protein, putative outer arm dynein light ch | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCTGGCGCATGGCGCATGGCGCGTGCTGCG |
Internal bar code: | GAGTCTTATTTACATTGCTAAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 828 |
LEAP-Seq percent confirming: | 27.7778 |
LEAP-Seq n confirming: | 30 |
LEAP-Seq n nonconfirming: | 78 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAAACATAATCGCAAAGGCG |
Suggested primer 2: | CTTGGTGTGTCCTCCCACTT |