| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.188860 |
| Chromosome: | chromosome 17 |
| Location: | 3823707 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g727900 | CRC1 | (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase; Centrin-related calmodulin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAACAGCTGGAGTCGGGGCAGCAGGAGTAG |
| Internal bar code: | GCGGGTCCCGAAATGTACGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 712 |
| LEAP-Seq percent confirming: | 99.8088 |
| LEAP-Seq n confirming: | 1044 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCAGCACCTCTTCCACAG |
| Suggested primer 2: | ACTTCGCTCAAACTGCTGCT |