| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.188868 |
| Chromosome: | chromosome 8 |
| Location: | 1870896 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g366900 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAGGCGTGATAACGAAGTCATGCGAGTT |
| Internal bar code: | AAGGGATAGGCGTAGTCGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1031 |
| LEAP-Seq percent confirming: | 99.88 |
| LEAP-Seq n confirming: | 7492 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCCCATCACACAACCAG |
| Suggested primer 2: | GCTTTGCGTTTAAGTTTCGC |