Insertion junction: LMJ.RY0402.188885_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.188885
Chromosome:chromosome 4
Location:3614823
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g228650 (1 of 18) 3.6.3.44 - Xenobiotic-transporting ATPase / Steroid-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTGAAGGCGAGGGGAGGGGTTGTGTTTGG
Internal bar code:GATTATTTCTGCCGGGGCTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:480
LEAP-Seq percent confirming:98.9186
LEAP-Seq n confirming:3110
LEAP-Seq n nonconfirming:34
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGTCGTACTCGACCACCT
Suggested primer 2:AAACAGAACCAACGGAAACG