Insertion junction: LMJ.RY0402.188885_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.188885
Chromosome:chromosome 4
Location:3862840
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g230242 MOT40 (1 of 1) IPR007914//IPR015422 - Uncharacterised protein family UPF0193 // Pyridoxal phosphate-dependent transferase, major region, subdomain 2; Predicted protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCACCGCCACCACCGGCTTTTCCAGCTTG
Internal bar code:CGATCAACTAACTACATAAATC

Confirmation - LEAP-Seq

LEAP-Seq distance:863
LEAP-Seq percent confirming:99.6577
LEAP-Seq n confirming:3203
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGCACCGAATAGGTCTGG
Suggested primer 2:CTGGCAAGGCGCTATTTAAG