Insertion junction: LMJ.RY0402.188915_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.188915
Chromosome:chromosome 9
Location:3642178
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g390356 (1 of 1) PTHR22849//PTHR22849:SF0 - WDSAM1 PROTEIN // WD REPEAT, SAM AND U-BOX DOMAIN-CONTAINING PROTEIN 1 intron
Cre09.g390393 (1 of 5) PF01713 - Smr domain (Smr) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTACTGCGTCGCTGCCTGCCAGTCGCCGCG
Internal bar code:ACATGAGGGACAATTTGCGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:707
LEAP-Seq percent confirming:99.511
LEAP-Seq n confirming:407
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTCGCTTCCTCAGTCTCCAC
Suggested primer 2:CCGCTGGTAGTACGTGTTCA