Insertion junction: LMJ.RY0402.188960_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.188960
Chromosome:chromosome 13
Location:1471125
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre13.g572375 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCAGCCCACACGCACACGCGCACACACA
Internal bar code:AAAAGTTGTCTGTGACATGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:965
LEAP-Seq percent confirming:43.8824
LEAP-Seq n confirming:642
LEAP-Seq n nonconfirming:821
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCGTTCTCTCTTTCGTCC
Suggested primer 2:CCTTGTGCAGCTTGAATGAA