Insertion junction: LMJ.RY0402.188965_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.188965
Chromosome:chromosome 9
Location:1869172
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g395600 OBGC,OBGC1 Factor possibly involved in assembly of the ribosomal 50S subunit; (1 of 1) PTHR11702:SF3 - GTP-BINDING PROTEIN 10 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCAATCATGGCCGGCTTGGTTTGGTTTAG
Internal bar code:CGTCATCACACAATCCGCACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:42
LEAP-Seq percent confirming:99.0991
LEAP-Seq n confirming:110
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AGTTTCCCAGTCACCTGTGG
Suggested primer 2:GACCCCGTCACCGACTACTA