| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.188997 |
| Chromosome: | chromosome_7 |
| Location: | 638291 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g316992 | sense | 3'UTR|outside_mRNA |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCACCCACTCAGACATGCAGGACCCCCTC |
| Internal bar code: | TGACTTGTGTCATAAGAATGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 428 |
| LEAP-Seq percent confirming: | 99.776 |
| LEAP-Seq n confirming: | 4900 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCAGAAGCACGCAATACT |
| Suggested primer 2: | GGCGTTTGTCCAGAAAACAT |