| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.189003 |
| Chromosome: | chromosome 12 |
| Location: | 8232357 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550400 | GRX2 | Glutaredoxin, CPYC type, cytosolic; (1 of 2) K03676 - glutaredoxin 3 (grxC, GLRX, GLRX2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCCGCACACGCTTGGCGTTTCACAGGT |
| Internal bar code: | GGGCCAGACGTGATACTAGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 721 |
| LEAP-Seq percent confirming: | 86.582 |
| LEAP-Seq n confirming: | 2523 |
| LEAP-Seq n nonconfirming: | 391 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTTCTTGCTAACCAGTCC |
| Suggested primer 2: | GCCTAAGAATTGATCGCTCG |