| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.189003 |
| Chromosome: | chromosome_12 |
| Location: | 8232383 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g550400 | GRX2 | Glutaredoxin, CPYC type | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCGCCAGGTTTGATGCAAAGACTAATGCAA |
| Internal bar code: | CGGACAAGCCCGAGATACGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 663 |
| LEAP-Seq percent confirming: | 99.7653 |
| LEAP-Seq n confirming: | 2550 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCAAGGTAGGATCGTCCG |
| Suggested primer 2: | CGCTTCTTGCTAACCAGTCC |