| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.189004 |
| Chromosome: | chromosome 16 |
| Location: | 4117591 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g669350 | (1 of 37) PF13639 - Ring finger domain (zf-RING_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACTGTAGCCTGCTGCCGGGCTCCCACCA |
| Internal bar code: | ACTTCTCGTTGTCTTGTGAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 142 |
| LEAP-Seq percent confirming: | 99.2189 |
| LEAP-Seq n confirming: | 4192 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | TCGCAATACTGCAAATGAGC |