| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.189007 |
| Chromosome: | chromosome 15 |
| Location: | 306727 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g635700 | MAPKKK12 | (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED; Mitogen-Activated Protein Kinase Kinase Kinase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTACTGTTGTTATGTACAGGCTGCAAAT |
| Internal bar code: | GCAATGGTGAGAAAATCCTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 792 |
| LEAP-Seq percent confirming: | 92.5466 |
| LEAP-Seq n confirming: | 1341 |
| LEAP-Seq n nonconfirming: | 108 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGTACGTAAAGCCAATCG |
| Suggested primer 2: | CAGCCCAAGCTAAACCAAAC |