Insertion junction: LMJ.RY0402.189011_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.189011
Chromosome:chromosome 6
Location:1792730
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g262400 (1 of 1) K14676 - lysophospholipid hydrolase (NTE, NRE) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCACGGCAACTCACGTACACACACGCACA
Internal bar code:CGGAAGGTCGCGGGATGGGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1
LEAP-Seq percent confirming:99.6732
LEAP-Seq n confirming:1525
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GAAACAGTCGTGTGGTGGTG
Suggested primer 2:CGCTGATCACCACATACAGG