Insertion junction: LMJ.RY0402.189016_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.189016
Chromosome:chromosome 1
Location:4041109
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g026300 (1 of 3) PF08238 - Sel1 repeat (Sel1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTAGGGATGGGGGCCCGGGGTTTGGCATC
Internal bar code:GCGCAGCGGCGACTTCTAAAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:389
LEAP-Seq percent confirming:99.9165
LEAP-Seq n confirming:1196
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTGACAGGAGAAGAAACGGC
Suggested primer 2:CTCTACAACCTGGCCACCAT