Insertion junction: LMJ.RY0402.189032_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.189032
Chromosome:chromosome 9
Location:3667209
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g390578 TRP23,FAP11 (1 of 6) PTHR10117 - TRANSIENT RECEPTOR POTENTIAL CHANNEL; Flagellar Associated Protein 11 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTCATGTTTATTACGTGGGTGTCACCACG
Internal bar code:TTTCCCGTAAACGAGGCCAGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:251
LEAP-Seq percent confirming:99.6968
LEAP-Seq n confirming:4933
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TGTCGGCCTTAGAGCAACTT
Suggested primer 2:CGCACATGGACACACATACA