Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.189036 |
Chromosome: | chromosome_2 |
Location: | 4967868 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g103950 | FAP100 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCATCTGCGCAGCCATCACCAACAGGAGAG |
Internal bar code: | CCGGAAATTGGACGCGAGCATA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 630 |
LEAP-Seq percent confirming: | 99.6675 |
LEAP-Seq n confirming: | 1199 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCACTAGCCGTCTTCAATG |
Suggested primer 2: | AGAACATGGCGGACTTCATC |