| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.189078 |
| Chromosome: | chromosome_12 |
| Location: | 7362836 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g557000 | APG12 | Autophagy-related protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAGTCCGGGAGATGTTCCTGGGTACCCTGC |
| Internal bar code: | CAGGGGGTGCGTGATAATTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 641 |
| LEAP-Seq percent confirming: | 71.7814 |
| LEAP-Seq n confirming: | 7092 |
| LEAP-Seq n nonconfirming: | 2788 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAGAGGGCAAGCTGCATGT |
| Suggested primer 2: | AGCAGAGCAAGCGAAGGTAG |