| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.189099 |
| Chromosome: | chromosome 3 |
| Location: | 7332154 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206650 | (1 of 1) PTHR24349:SF88 - SERINE/THREONINE-PROTEIN KINASE CG17528 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTTGGTGACGGAGTTGGACTTCTTGATT |
| Internal bar code: | CCACGCTGCGTTTATTCAACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 623 |
| LEAP-Seq percent confirming: | 99.1729 |
| LEAP-Seq n confirming: | 3597 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTTCCATATTAGCAGCCA |
| Suggested primer 2: | TGTTTCTCAGGCATGCTCAC |