Insertion junction: LMJ.RY0402.189101_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.189101
Chromosome:chromosome 9
Location:4463330
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g395695 (1 of 52) 2.7.11.22 - Cyclin-dependent kinase / Cdk-activating protein kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTTGCAAAGTCGCCGCCACTCGTCAACA
Internal bar code:CTATACATTGCACCCGCGCCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:688
LEAP-Seq percent confirming:98.4412
LEAP-Seq n confirming:1263
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AGACGTCGGCTAACGATTTG
Suggested primer 2:AACTTGAGCCTGCTCATTGG