Insertion junction: LMJ.RY0402.189165_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.189165
Chromosome:chromosome 10
Location:4605103
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g452750 TPT18,TPT16 UMAMIT (Usually Multiple Acids Move In and Out Transporters) type transporter; (1 of 3) PTHR11132//PTHR11132:SF125 - SOLUTE CARRIER FAMILY 35 // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATGTCGCGTGCATCCATGCCCGTGCTCAC
Internal bar code:CTCGAGCCAAGGGCTGGCAGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:367
LEAP-Seq percent confirming:99.5
LEAP-Seq n confirming:597
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CATCTTTCAAGGTGTGTGCG
Suggested primer 2:CCCCTATATTGCATGGATGG