| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.189204 |
| Chromosome: | chromosome 6 |
| Location: | 3597613 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278123 | VPS39 | (1 of 781) IPR000104 - Antifreeze protein, type I; Subunit of VPS-C complex | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCAAGCCACACACGTGGACTTGCTGCG |
| Internal bar code: | CGCACTGTTAGTCCCGGTTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 244 |
| LEAP-Seq percent confirming: | 99.7139 |
| LEAP-Seq n confirming: | 2440 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAGCAGCATGAGGAGCTG |
| Suggested primer 2: | TTACATTTGAGCAACGCCTG |