Insertion junction: LMJ.RY0402.189217_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.189217
Chromosome:chromosome_2
Location:3653493
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre02.g095149 FAP143 Flagellar Associated Protein antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GTGGTGATGTGTGCTGGCATAGTCGGACAA
Internal bar code:TAATTTCATCGTGTGGCGTGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1006
LEAP-Seq percent confirming:96.765
LEAP-Seq n confirming:1705
LEAP-Seq n nonconfirming:57
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TTGGAATGTGTGTGTTGGCT
Suggested primer 2:GACTACGGGCACTGAAGCTC