| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.189227 |
| Chromosome: | chromosome 12 |
| Location: | 5938232 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534350 | HCD2 | (1 of 1) 1.1.1.157 - 3-hydroxybutyryl-CoA dehydrogenase / BHBD; 3-hydroxyacyl-CoA dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTTCCATGACCCTCCCCGGCTTGTCCGC |
| Internal bar code: | GAATGCGCCGCCCGGAGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 203 |
| LEAP-Seq percent confirming: | 85.4247 |
| LEAP-Seq n confirming: | 3118 |
| LEAP-Seq n nonconfirming: | 532 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCCAGTGATTACAGTCAA |
| Suggested primer 2: | TTCAATCCCCCTCCTTATCC |