| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.189229 |
| Chromosome: | chromosome 6 |
| Location: | 4174914 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278231 | (1 of 19) K08850 - aurora kinase, other [EC:2.7.11.1] (AURKX) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTTCTGGCTCGTTTGCTTTGATTCAGGC |
| Internal bar code: | ACGCCCGGCCGTCCCATTCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 482 |
| LEAP-Seq percent confirming: | 99.7323 |
| LEAP-Seq n confirming: | 7450 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGTGACATTGTTCCGTGA |
| Suggested primer 2: | CCAGCAAATCCACATCACAC |