| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.189244 |
| Chromosome: | chromosome 9 |
| Location: | 3946500 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392430 | (1 of 1) 1.1.1.62 - 17-beta-estradiol 17-dehydrogenase / Estrogen 17-oxidoreductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGGCCAGCTGGTCCCAAAGTATCTGCC |
| Internal bar code: | GAGTCCTAGGGGTTTCGATGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 911 |
| LEAP-Seq percent confirming: | 93.6747 |
| LEAP-Seq n confirming: | 1555 |
| LEAP-Seq n nonconfirming: | 105 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGGCTGATTACACGGAAC |
| Suggested primer 2: | CCCAAAACTGCACAACCTTT |