| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.189281 |
| Chromosome: | chromosome 15 |
| Location: | 269627 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g640900 | (1 of 17) IPR029069 - HotDog domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGAAGCCTTGGAGCCGTGCTGGCGACCG |
| Internal bar code: | GCTGCAGTGAACGAGTCTCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 895 |
| LEAP-Seq percent confirming: | 85.273 |
| LEAP-Seq n confirming: | 3202 |
| LEAP-Seq n nonconfirming: | 553 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCTACCGAGTTCCTCAG |
| Suggested primer 2: | AGCAGACGTGAGGATTGCTT |