Insertion junction: LMJ.RY0402.189283_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.189283
Chromosome:chromosome 17
Location:6319492
Confidence (%):58
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTTGCGTCGCTCTTATTCTGGGACGACTG
Internal bar code:GTAGGTGGTTCGTCGCATGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:244
LEAP-Seq percent confirming:82.8542
LEAP-Seq n confirming:807
LEAP-Seq n nonconfirming:167
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CATAGGAATGCAGCTGAGCA
Suggested primer 2:GCGATGCCATAGCAACTGTA