| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.189289 |
| Chromosome: | chromosome 3 |
| Location: | 1707349 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g153400 | (1 of 1) 3.3.2.9 - Microsomal epoxide hydrolase / Microsomal epoxide hydratase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCAAATCCACTTTCAGAATTTCAATCAG |
| Internal bar code: | GAGGCTTTGGGCTGATAGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 364 |
| LEAP-Seq percent confirming: | 96.1432 |
| LEAP-Seq n confirming: | 1047 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGTATGACCGGACGAAGG |
| Suggested primer 2: | TTGCTTCACTTGCTTGTTGG |