| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.189293 |
| Chromosome: | chromosome 9 |
| Location: | 7445700 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g413500 | GT90-16,GT90F16 | (1 of 4) PTHR12203//PTHR12203:SF35 - KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED // SUBFAMILY NOT NAMED; GT90 family protein 16 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGAAGGCCACCAAGCTCCATGCTACAAT |
| Internal bar code: | GCATCACCTCCTGCACGCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 333 |
| LEAP-Seq percent confirming: | 92.5 |
| LEAP-Seq n confirming: | 518 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCCTTTTTCGGAAATCT |
| Suggested primer 2: | GCATGGTACCAACCCTGTCT |