| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.189351 |
| Chromosome: | chromosome 9 |
| Location: | 1302859 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399073 | (1 of 2) PTHR14209:SF9 - ISOAMYL ACETATE-HYDROLYZING ESTERASE 1 HOMOLOG | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGACTGTTCGAGGTACCCGTACCAGCTC |
| Internal bar code: | GCCGGACGAGCTGCCGGGCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 176 |
| LEAP-Seq percent confirming: | 99.8475 |
| LEAP-Seq n confirming: | 1964 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACTCGATGTCTCTTGCCA |
| Suggested primer 2: | AGGATACCCCATCCTTCACC |