| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.189385 |
| Chromosome: | chromosome_1 |
| Location: | 2648125 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g015500 | CGL91 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACACCTCAATTCAGCCGTCCTGCAAGACTC |
| Internal bar code: | GGTTCCAAGACCATATCGATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 111 |
| LEAP-Seq percent confirming: | 98.8095 |
| LEAP-Seq n confirming: | 83 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAGACGGATGATGGTGTG |
| Suggested primer 2: | CACACCTTCCACCCTGTCTT |