Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.189385 |
Chromosome: | chromosome_1 |
Location: | 2648129 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g015500 | CGL91 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTGTTCTGCTCTCAAGCATACATGGTACAT |
Internal bar code: | TCACGGTTACCTCGGGGGTGGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 840 |
LEAP-Seq percent confirming: | 99.1364 |
LEAP-Seq n confirming: | 6658 |
LEAP-Seq n nonconfirming: | 58 |
LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACACCTTCCACCCTGTCTT |
Suggested primer 2: | GACAGACGGATGATGGTGTG |